53BP1(Phospho-Thr543) Rabbit Polyclonal Antibody

SKU:BHA12103500
Overview
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TP53BP1-specific polyclonal antibody raised in Rabbit. Validated for Western Blot in Human, Rat, Mouse samples. Phospho-specific. Affinity-purified rabbit IgG.
Specificity Phospho-Specific
Immunogen Synthesized phosho peptide around human 53BP1 (Thr543)
UniProt Q12888
Gene ID Human: 7158; Mouse: 27223
Applications 1 validated
Available Options
Size
Options selector
Catalog no. Size
BT-AP00165-20UL 20 uL
BT-AP00165-50UL 50 uL
BT-AP00165-100UL 100 uL
Field Specification
Alternative Names 53BP1; Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID Human: 7158; Mouse: 27223
Host Rabbit
Immunogen Synthesized phosho peptide around human 53BP1 (Thr543)
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Rat
  • Mouse
Storage -20°C for 1 year
Target TP53BP1

Product Overview

Primary Antibody

This antibody targets TP53BP1 and detects endogenous levels of TP53BP1 protein. Only detects the phosphorylated form of TP53BP1.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target TP53BP1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human, Rat, Mouse
Purification Affinity Purification
Concentration 1 mg/ml
Immunogen Synthesized phosho peptide around human 53BP1 (Thr543)
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -20°C for 1 year
Gene ID Human: 7158; Mouse: 27223
UniProt Q12888 (Human: Q12888; Mouse: P70399)

Alternative Names

53BP1; Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetTP53BP1
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman, Rat, Mouse
PurificationAffinity Purification
Concentration1 mg/ml
ImmunogenSynthesized phosho peptide around human 53BP1 (Thr543)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20°C for 1 year
Gene IDHuman: 7158; Mouse: 27223
UniProtQ12888 (Human: Q12888; Mouse: P70399)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution

Alternative Names

53BP1; Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1

What species does this antibody react with?
This antibody has been validated for reactivity against Human, Rat, Mouse. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB). Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of TP53BP1?
The expected molecular weight of TP53BP1 can be found in the product datasheet. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.
Is this antibody specific for the phosphorylated form of TP53BP1?
Yes. This antibody is designed to detect TP53BP1 only at the phosphorylation site specified in the product name. Lambda phosphatase treatment eliminates the signal, confirming phospho-specificity.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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