ABCA4 Antibody / ATP-binding cassette sub-family A member 4

Overview

ABCA4 Antibody / ATP-binding cassette sub-family A member 4 is an antibody targeting ABCA4, raised in Goat for protein detection and localization studies where these specifications are required.

Key elements and design rationale

• Target: ABCA4 (reported localization: Membrane, ER, Cytoplasmic vesicles).
• Antibody identity: Polyclonal (goat origin); Goat Ig.
• Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
• Format: Antigen affinity purified.
• Species reactivity: Human, Mouse.
• Listed applications: IF, ELISA (peptide) (refer to on-page specifications for application-specific guidance).

Biological background

The ABCA4 protein is a transporter molecule responsible for moving retinal molecules across cell membranes in the eye and may have a broader impact on neurological processes. Research has shown that this protein is not restricted to the eyes, but is also present in various regions of the brain. ABCA4 plays a role in the maintenance of lipid homeostasis in the central nervous system. Lipids are essential components of cell membranes and are crucial for neuronal function. Disruptions in lipid metabolism have been implicated in numerous neurological disorders, including Alzheimer's disease and Parkinson's disease. ABCA4 has been found to interact with other proteins involved in synaptic transmission and neuronal signaling. This suggests that the protein may play a role in modulating communication between neurons, which is essential for cognitive function.

Research relevance and current trends

• Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
• Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
• Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.

Common research applications

• Immunofluorescence: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
• ELISA: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.

Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.

Notes for experimental interpretation

• Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
• Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
• Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
• Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.