| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ABCE1 recombinant protein (Position: Q141-D599) was used as the immunogen for the ABCE1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ABCE1 Antibody / ATP-binding cassette sub-family E member 1 is a anti-ABCE1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: ABCE1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, ICC, WB
Biological background
Structurally, ABCE1 is a 599-amino-acid protein of approximately 68 kilodaltons composed of two nucleotide-binding domains (NBDs) and an N-terminal region containing iron-sulfur clusters critical for its activity. These clusters facilitate conformational changes required for ribosome disassembly. ABCE1 interacts with release factors (eRF1 and eRF3) and ribosomal subunits, coupling ATP hydrolysis to subunit separation. The protein is ubiquitously expressed and localized in the cytoplasm, with enrichment near polysomes.
The ABCE1 antibody is widely used in molecular biology, virology, and translational control research to study ribosome dynamics, translation termination, and antiviral mechanisms. Western blot analysis detects a 68 kilodalton band corresponding to ABCE1, while immunofluorescence shows diffuse cytoplasmic distribution associated with ribosomal clusters. This antibody enables exploration of how ATPase-driven ribosome recycling maintains translation efficiency and proteostasis.
Functionally, ABCE1 acts as a �ribosome recycling factor,� splitting 80S ribosomes into 40S and 60S subunits after peptide release. It also inhibits RNase L activation, thereby modulating innate antiviral responses. In viruses such as HIV-1, ABCE1 facilitates Gag assembly, linking translation control to viral replication. The ABCE1 gene is essential for cell viability, and its disruption leads to impaired translation and cell death. The ABCE1 antibody provides a powerful tool for studying the intersection of translation regulation, ribosome turnover, and antiviral defense.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
