| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ABCG5 recombinant protein (Position: M1-S88) was used as the immunogen for the ABCG5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ABCG5 Antibody / ATP-binding cassette sub-family G member 5 is a anti-ABCG5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ABCG5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IF, IP
Biological background
Structurally, ABCG5 is a 651-amino-acid integral membrane glycoprotein of approximately 70 kilodaltons that contains one nucleotide-binding domain (NBD) and one transmembrane domain (TMD). It requires heterodimerization with ABCG8 for stability and activity, forming a functional exporter that utilizes ATP hydrolysis to transport sterols across lipid bilayers. ABCG5 is highly expressed in liver, small intestine, and gallbladder epithelium, where it contributes to cholesterol homeostasis and xenosterol elimination.
The ABCG5 antibody is widely used in lipid metabolism, hepatology, and cardiovascular research to study sterol transport, bile secretion, and cholesterol regulation. Western blot analysis detects a 70 kilodalton band corresponding to ABCG5, while immunofluorescence reveals localization to apical plasma membranes of enterocytes and canalicular membranes of hepatocytes. This antibody enables detailed investigation of sterol transport mechanisms and lipid trafficking defects underlying metabolic disorders.
Loss-of-function mutations in ABCG5 cause sitosterolemia, a rare lipid disorder characterized by excessive absorption and accumulation of dietary plant sterols and premature atherosclerosis. Conversely, increased ABCG5 expression contributes to enhanced sterol efflux and protection against hypercholesterolemia. The ABCG5/ABCG8 complex is also regulated by nuclear receptors such as liver X receptor (LXR) and farnesoid X receptor (FXR), integrating lipid metabolism with transcriptional control. The ABCG5 antibody provides a high-quality reagent for monitoring transporter expression, assessing sterol efflux activity, and exploring therapeutic regulation of cholesterol metabolism.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
