| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ABR recombinant protein (Position: Q180-V859) was used as the immunogen for the ABR antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ABR Antibody / Active breakpoint cluster region-related protein is a anti-ABR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: ABR
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
ABR is encoded by the ABR gene located on human chromosome 17p13.3. The protein is approximately 871 amino acids long and contains a GTPase-activating (GAP) domain and a guanine nucleotide exchange factor (GEF)-like domain, giving it dual regulatory capacity. ABR localizes to the cytoplasm and plasma membrane, where it modulates actin cytoskeleton remodeling and cell polarity.
The ABR antibody detects a 98 kilodalton protein by western blot and shows perinuclear and membrane-associated staining under immunofluorescence. ABR acts as a negative regulator of Rac and Cdc42 by promoting their conversion from active GTP-bound to inactive GDP-bound states. This modulation is essential for neuronal growth cone collapse, immune cell migration, and epithelial polarity.
In neurons, ABR contributes to axon guidance and dendritic spine development, while in immune cells it regulates phagocytosis and chemotaxis. Loss of ABR activity leads to hyperactivation of Rho GTPases, resulting in abnormal morphology and impaired signal transduction. In cancers, aberrant ABR expression alters cell adhesion, invasion, and metastatic behavior.
Beyond cytoskeletal control, ABR interacts with signaling molecules involved in inflammation and oxidative stress. It participates in cellular defense mechanisms by modulating NADPH oxidase activity and reactive oxygen species production. These diverse functions establish ABR as a central hub linking signaling to structural adaptation.
As a bidirectional regulator of GTPase signaling, ABR provides key insights into cell dynamics and tissue morphogenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
