| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human ACADVL/VLCAD was used as the immunogen for the ACADVL antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ACADVL Antibody / VLCAD is a anti-ACADVL Rabbit antibody Recombinant Rabbit Monoclonal clone 32A31 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Human.
Key elements and design rationale
- Target: ACADVL
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32A31, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, IP
Biological background
ACADVL antibody is an important tool in metabolic and mitochondrial research. Deficiency of VLCAD due to mutations in the ACADVL gene leads to very long-chain acyl-CoA dehydrogenase deficiency, an inherited metabolic disorder characterized by hypoglycemia, cardiomyopathy, and muscle weakness. By detecting VLCAD expression and localization, researchers can investigate the molecular basis of fatty acid oxidation disorders and explore therapeutic approaches.
Applications of ACADVL antibody include western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. In western blot assays, the antibody specifically detects VLCAD protein, enabling comparison of expression across tissues and experimental conditions. Immunohistochemistry provides mitochondrial localization patterns in tissue sections, while immunofluorescence highlights subcellular distribution in cultured cells. These applications make the antibody useful for both clinical research and basic studies of metabolism.
In addition to its clinical relevance, VLCAD has been linked to metabolic regulation in cancer and cardiovascular disease. Altered fatty acid oxidation is a hallmark of many cancers, and modulation of VLCAD activity influences tumor growth and metabolic reprogramming. In cardiology, VLCAD function is crucial for sustaining energy production in heart tissue, particularly under stress. Using ACADVL antibody, researchers can study how VLCAD expression correlates with disease progression and therapeutic outcomes.
Beyond disease-focused research, ACADVL antibody supports investigations into exercise physiology, fasting metabolism, and mitochondrial adaptation. By monitoring VLCAD activity, scientists gain insights into how energy metabolism adjusts under varying nutritional and physiological states. This makes the antibody valuable for a wide range of experimental designs.
ACADVL antibody offered by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
