| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ACE recombinant protein (Position: R149-C545) was used as the immunogen for the ACE antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ACE Antibody / Angiotensin I Converting Enzyme is a anti-ACE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ACE
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Functionally, ACE antibody identifies a 1,306-amino-acid type I membrane glycoprotein composed of two homologous catalytic domains (N- and C-domains) and a short cytoplasmic tail. ACE is expressed in endothelial cells, epithelial cells, and kidney proximal tubules, where it acts as a key enzyme of the renin-angiotensin system (RAS). By generating angiotensin II, ACE promotes vasoconstriction, sodium retention, and aldosterone secretion, contributing to blood pressure regulation.
The ACE gene is located on chromosome 17q23.3 and encodes multiple isoforms, including somatic ACE and testis-specific ACE (tACE). Somatic ACE is primarily found on the luminal surface of endothelial cells in lungs, heart, and kidneys, whereas tACE functions in sperm maturation and fertility. ACE expression is regulated by inflammatory cytokines, hormonal signaling, and mechanical stress on vascular endothelium.
Clinically, dysregulation of ACE activity is linked to hypertension, heart failure, diabetic nephropathy, and atherosclerosis. ACE inhibitors, which block enzyme activity, are among the most effective therapeutics for cardiovascular disease management. Beyond cardiovascular roles, ACE contributes to immune function by degrading inflammatory peptides and influencing hematopoietic stem cell mobilization. Elevated ACE levels are also diagnostic for sarcoidosis and other granulomatous disorders.
ACE antibody is widely used in cardiovascular, renal, and molecular physiology research. It is suitable for immunohistochemistry, western blotting, and flow cytometry to detect ACE expression and distribution in tissues. This antibody supports studies of vascular biology, RAS signaling, and peptide metabolism. In clinical and translational settings, ACE serves as a biomarker for endothelial activation and inflammatory vascular disease.
Structurally, ACE consists of two catalytic domains containing zinc-binding motifs (HEMGH sequence) that coordinate enzymatic hydrolysis of peptide bonds. Its extracellular domain is heavily glycosylated, conferring stability and resistance to proteolysis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
