| Field | Specification |
|---|---|
| Alternative Names | FO108;H4;H4/n;H4F2;H4FN;HIST2H4;Histone H4;HIST1H4A;HIST2H4A |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | A synthetic acetylated peptide around K8 of human Histone H4(NP_003529.1). |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Acetylated Antibodies
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | See datasheet |
| Immunohistochemistry (IHC) | See datasheet |
| IF / ICC | See datasheet |
| Immunoprecipitation (IP) | See datasheet |
| ChIP | See datasheet |
| ChIP-seq | See datasheet |
Product Specifications
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Species Reactivity | Human, Mouse, Rat, Other, (Wide, Range) |
| Conjugate | Unconjugated |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 11 kDa |
| Modification | Acetylated |
| UniProt / Swiss-Prot | P62805 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Immunogen / Antigen
A synthetic acetylated peptide around K8 of human Histone H4(NP_003529.1).
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for WB, IHC, IF/ICC, IP, ChIP, ChIP-seq. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Mouse, Rat, Other, (Wide, Range). Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Rabbit. For indirect detection (WB, IHC, IF), select an anti-Rabbit secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
A synthetic acetylated peptide around K8 of human Histone H4(NP_003529.1).
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Mouse, Rat, Other, (Wide, Range) samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
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