| Field | Specification |
|---|---|
| Detection Method | |
| Product Type | |
| Sample Type(s) | whole blood, serum, plasma etc |
| Shipping | |
| Species | |
| Storage |
Overview
Direct assays of acetylcholinesterase activity in blood, serum, plasma, and other biological samples. The assay uses Ellman method, OD410nm for signal readout. Compatible sample input includes whole blood, serum, plasma etc. Typical stated assay timing is 10 min.
Key elements and design rationale
- Readout format: Ellman method, OD410nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes whole blood, serum, plasma etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 6 U/L for interpreting low-signal samples.
- Feature emphasis: Simple and Convenient. This single reagent is ready to use, when reconstituted in 25mL deionized water.
Additional feature notes highlight High-throughput. Can be readily adapted on chemistry analyzers or high-throughput liquid handling system to test thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of acetylcholinesterase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
ACETYLCHOLINESTERASE(EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. Low serum cholinesterase activity may relate to exposure to insecticides or to one of a number of variant genotypes. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Cholinesterase levels of cells and plasma are used as a guide in establishing safety precautions relative to exposure and contact, as well as a guide in determining the need for workers to be removed from areas of contact with the organic phosphate insecticides.
Simple, direct and automation-ready procedures for measuring AChE activity are very desirable. BioAssay Systems’ acetylcholinesterase reagent is based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5′-dithiobis(2-nitrobenzoic acid). The intensity of the product color at 410 nm is proportionate to the enzyme activity in the sample.
Detection method
Colorimetric (OD 410 nm).
Detection limit and analytical sensitivity
Reported detection limit: 6 U/L.
Procedures and timing
Stated procedure or timing information: 10 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Prepare control-treated samples for paired use with compatible assay-kit plates.
- Benchmark assay response against defined control conditions in replicate wells.
- Optimize reagent concentration and incubation windows for plate-based comparisons.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What is the difference between this reagent and your DACE-100 assay kit?
This is a single reagent that has been optimized for use on both Chemistry Analyzer and in 96-well plate assay systems. The reagent is convenient and before use, only needs to mix with 25mL distilled or deionized water.
Does the assay detect only acetylcholinesterase activty?
No, this assay detects acetylcholinesterase and butyrylcholinesterase (also known as plasma cholinesterase, pseudocholinesterase) and does not distinguish between the two.
Which anticoagulant should I use for blood sample collection?
We recommend using serum for measuring plasma cholinesterase activity and EDTA blood for measuring red blood cell acetylcholinesterase activity.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
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