| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ACP2 recombinant protein (Position: R31-H88) was used as the immunogen for the ACP2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ACP2 Antibody / Lysosomal acid phosphatase 2 is a anti-ACP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ACP2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
ACP2 is encoded by the ACP2 gene located on human chromosome 11p11.2. The protein is approximately 452 amino acids in length and is synthesized as a precursor that undergoes proteolytic cleavage into two subunits�a 36 kilodalton alpha subunit and a 14 kilodalton beta subunit�that together form the active enzyme. ACP2 localizes predominantly to the lysosomal lumen but can also be detected in late endosomes and phagosomes, reflecting its involvement in diverse degradative pathways.
The ACP2 antibody detects the 50 kilodalton precursor and its processed subunits by western blot and shows punctate lysosomal staining by immunofluorescence microscopy. ACP2 participates in the hydrolysis of phosphate-containing substrates derived from nucleotides, phospholipids, and other macromolecules, releasing inorganic phosphate for metabolic reuse. It complements the function of acid phosphatase ACP3, ensuring redundancy in phosphate metabolism and cellular cleanup.
Mutations or deficiencies in ACP2 lead to acid phosphatase deficiency, a rare lysosomal storage disorder characterized by abnormal accumulation of phosphomonoesters and progressive neurodegeneration. Reduced ACP2 activity has also been implicated in osteopetrosis-like bone disorders and altered immune responses, as lysosomal enzymes regulate antigen presentation and macrophage function. Conversely, upregulation of ACP2 is observed during macrophage activation and tissue remodeling, highlighting its adaptive role in immune and metabolic balance.
As a lysosomal hydrolase linking phosphate metabolism to intracellular clearance, ACP2 is a valuable marker for lysosome integrity and metabolic health.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
