| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide specific to human Smooth muscle actin was used as the immunogen for the ACTA2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ACTA2 Antibody / Alpha Actin / Smooth Muscle Actin is a anti-ACTA2 Rabbit antibody Recombinant Rabbit Monoclonal clone EO-1 supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P, Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic.
Key elements and design rationale
- Target: ACTA2
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone EO-1, isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB, IHC-P, IF
Biological background
Alpha actin is abundantly expressed in smooth muscle cells of the vasculature, airways, gastrointestinal tract, and reproductive system. It is also used as a marker of myofibroblast differentiation, where its upregulation reflects tissue remodeling during wound healing, fibrosis, and pathological scarring. Because of its strong and specific expression pattern, detection with ACTA2 antibody is widely applied in immunohistochemistry and immunofluorescence to identify smooth muscle or myofibroblastic cells in tissue samples.
In cardiovascular research, ACTA2 is studied as a marker of vascular smooth muscle cells and their phenotypic switching. Under pathological conditions such as atherosclerosis or hypertension, ACTA2 expression can change as smooth muscle cells adopt synthetic phenotypes. In pulmonary and hepatic fibrosis, ACTA2 positive myofibroblasts contribute to extracellular matrix deposition and tissue stiffening, making ACTA2 a reliable indicator of fibrotic remodeling.
Structurally, alpha actin is a globular protein that polymerizes into filamentous actin, forming part of the cytoskeleton. ACTA2 interacts with actin binding proteins to regulate filament dynamics, cell shape, and motility. Its high conservation across species ensures that ACTA2 antibody is effective in many experimental systems, including human and animal models.
The ACTA2 antibody is commonly used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. These applications enable researchers to assess expression, distribution, and structural changes of smooth muscle actin in both healthy and diseased tissues. For scientists investigating vascular biology, fibrosis, tissue remodeling, or smooth muscle physiology, the ACTA2 antibody provides a sensitive and specific detection tool.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
