| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human ACTR1B was used as the immunogen for the ACTR1B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ACTR1B Antibody / Actin related protein 1B is a anti-ACTR1B Rabbit antibody Recombinant Rabbit Monoclonal clone 30A59 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: ACTR1B
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30A59, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
ACTR1B antibody is widely applied in neuroscience, cell biology, and developmental biology. By detecting ACTR1B, researchers can evaluate how dynein dynactin interactions support intracellular transport. This process is essential for neuronal function, where long distance axonal transport delivers synaptic vesicles and organelles. Mutations or disruption of dynactin subunits, including ACTR1B, impair axonal transport and cause neurodegenerative disease. ACTR1B expression is detected in brain, muscle, and immune cells, reflecting its broad roles in intracellular logistics.
Applications of ACTR1B antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects ACTR1B protein in neuronal lysates, immunohistochemistry maps expression in brain sections, and immunofluorescence reveals localization along microtubules and vesicular cargo. These approaches allow direct visualization of dynactin function in cellular transport.
Dynactin acts as a cofactor for dynein in multiple processes, including mitotic spindle orientation, endosome trafficking, and immune synapse formation. By applying ACTR1B antibody, scientists can study how transport machinery coordinates cell division, signaling, and migration. In developmental biology, ACTR1B is required for correct neuronal wiring and tissue morphogenesis, as transport defects disrupt growth cone guidance and organelle distribution.
Aberrant dynactin activity contributes to neurodegenerative diseases such as amyotrophic lateral sclerosis and hereditary spastic paraplegia. While most mutations involve the p150Glued subunit, ACTR1B remains essential for filament integrity. Detection with ACTR1B antibody provides a foundation for exploring dynactin biology in disease contexts. In cancer, altered microtubule transport and polarity also involve dynactin regulation, linking ACTR1B studies to oncology.
ACTR1B antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
