| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ADGRE5 recombinant protein (Position: R287-Q690) was used as the immunogen for the ADGRE5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ADGRE5 Antibody / Adhesion G protein-coupled receptor E5 / CD97 is a anti-ADGRE5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: ADGRE5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
ADGRE5 undergoes autoproteolytic cleavage within the GAIN domain, producing a large extracellular alpha subunit and a smaller membrane-bound beta subunit that remain noncovalently associated. This processing is essential for receptor maturation and ligand binding. The EGF-like domains mediate interaction with CD55, linking ADGRE5 function to complement regulation and immune evasion. In immune cells, ADGRE5 participates in leukocyte migration to inflammatory sites, co-stimulation of T cells, and macrophage activation. It also contributes to angiogenesis, smooth muscle contraction, and cell-cell adhesion in epithelial tissues. Dysregulated expression of CD97 has been associated with inflammatory disorders and several cancers, where it promotes invasion, epithelial-mesenchymal transition, and metastasis.
The ADGRE5 antibody is widely used in immunology, cancer biology, and vascular research to detect receptor expression and distribution. Western blot analysis identifies multiple bands (75-120 kilodaltons) corresponding to the various glycosylated receptor forms, while flow cytometry and immunohistochemistry reveal strong membrane localization. In tumors, overexpression of ADGRE5 enhances motility through interactions with integrins and activation of RhoA and MAPK pathways. Elevated expression correlates with poor prognosis in colorectal, thyroid, and gastric cancers. Conversely, in leukocytes, ADGRE5 expression is dynamically regulated during activation and differentiation, serving as a marker for immune cell subsets.
Beyond cancer and inflammation, ADGRE5 participates in vascular remodeling and smooth muscle physiology. It mediates heterotypic interactions between leukocytes and endothelial cells, facilitating transmigration across the vessel wall. Experimental deletion of ADGRE5 in mice reduces inflammatory cell recruitment and limits tissue damage, underscoring its role in immune regulation. The ADGRE5 antibody enables detailed characterization of receptor expression and function across tissues and disease contexts.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
