| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | AE binding protein 2, AEBP2, Adipocyte Enhancer binding protein 2 |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human Adipocyte Enhancer binding protein 2 (AEBP2), GenBank Accession No. NM_153207, a.a. 2-503 (end) with N-terminal His-tag, MW=53.6 kDa, expressed in Sf9 cells via a baculovirus expression system. Runs at ~70 kDa on SDS-PAGE gels. *Note: Protein runs aberrantly in SDS-PAGE gels, and has been confirmed by Western blotting.
Product Description
Insect Cells (Sf9) ExpressionRecombinant AEBP2 Human protein is produced using a validated Insect Cells (Sf9) expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | Q6ZN18 |
|---|---|
| Expression System | Insect Cells (Sf9) |
| Amino Acids / Region | 2-503 (end) |
| Affinity Tag | N-terminal His-tag |
| Molecular Weight | 53.6 kDa |
| Formulation | 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl and 20% glycerol |
| Storage | At least 6 months at -80°C. |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles.
This protein was produced in Insect Cells (Sf9). Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 2-503 (end). Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Useful for binding studies to investigate protein-protein interactions in the EZH2 complex. Also suitable as an immunogen or as a standard for Western blotting. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
At least 6 months at -80°C. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Kim, H., et al., PLoS One. 2011; 6(9):e25174.
- Cao., R., et al., Mol. Cell. 2004 Jul 2; 15(1): 57-67.
