| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human AHCY recombinant protein (Position: W112-Y432) was used as the immunogen for the AHCY antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
AHCY Antibody / Adenosylhomocysteinase is a anti-AHCY Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: AHCY
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Structurally, AHCY is a homotetrameric enzyme that binds NAD+ as a cofactor to mediate the reversible breakdown of SAH. It belongs to the adenosylhomocysteinase family of dehydrogenases, characterized by a conserved catalytic lysine and nicotinamide-binding motif. The enzyme undergoes conformational changes during catalysis, coupling the oxidation and hydrolysis of SAH to the regeneration of adenosine and homocysteine. AHCY operates at the intersection of methionine metabolism, DNA methylation, and nucleotide synthesis.
Functionally, AHCY plays a central role in controlling cellular methylation capacity by regulating the intracellular SAH concentration, a potent inhibitor of methyltransferases. By hydrolyzing SAH, AHCY maintains the S-adenosylmethionine (SAM)/SAH ratio required for normal methylation of DNA, RNA, proteins, and lipids. In liver, AHCY supports homocysteine recycling and methionine regeneration, while in neurons, it contributes to methylation-dependent neurotransmitter synthesis. The enzyme also interacts with metabolic partners such as MAT1A and CBS, forming a regulatory network that maintains one-carbon metabolism.
Mutations or deficiency in AHCY cause adenosylhomocysteinase deficiency, a rare metabolic disorder characterized by elevated SAH and global hypomethylation leading to developmental delay, muscle hypotonia, and hepatic dysfunction. Dysregulation of AHCY expression has also been linked to cardiovascular disease, liver fibrosis, and cancer. Pathway involvement includes methionine metabolism, homocysteine clearance, and methyl donor recycling. During development, AHCY expression ensures proper methylation patterns necessary for organogenesis and epigenetic regulation.
The AHCY antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
