| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Aldolase C/ALDOC was used as the immunogen for the ALDOC antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ALDOC Antibody / Aldolase C is a anti-ALDOC Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, extracellular.
Key elements and design rationale
- Target: ALDOC
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): IHC, WB
Biological background
Structurally, Aldolase C functions as a homotetramer with catalytic lysine residues that form Schiff bases with its substrate. It shares high sequence identity with Aldolase A (muscle type) and B (liver type) but exhibits unique kinetic properties suited for neuronal metabolism. In neurons and glial cells, Aldolase C contributes to glucose utilization, energy coupling, and maintenance of synaptic function. It also interacts with cytoskeletal proteins, suggesting roles beyond metabolism, including regulation of actin organization and cell morphology.
The ALDOC antibody is widely used in neuroscience, metabolism, and cell biology research to study glycolytic regulation and neural differentiation. Western blot analysis identifies a 39 kilodalton band corresponding to Aldolase C, while immunohistochemistry reveals strong cytoplasmic staining in Purkinje cells and astrocytes. This antibody supports investigation of metabolic compartmentalization and energy flux in the central nervous system.
Altered expression of Aldolase C has been observed in neurodegenerative diseases and gliomas, where metabolic reprogramming contributes to pathogenesis. Beyond the brain, ALDOC expression in epithelial and endocrine tissues suggests additional roles in energy balance and biosynthesis. The ALDOC antibody provides a reliable reagent for analyzing glycolytic enzyme distribution and function.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
