Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Rabbit]

SKU:BHA10908464
Suppliers
Applied Biological Materials (abm) Inc.
Applied Biological Materials (abm) Inc.
Details Products
Overview
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Anti-Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Rabbit] antibody. Reactive against Mouse targets.
Species Reactivity Mouse
Options selector
Catalog no. Size
SA024 250 ug
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 250 ug
  • Lead time: In Stock at Manufacturer options listed in “Availability Content”; other statuses may take longer.
  • Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No SA024
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Immunogen Mouse IgG whole molecule
Product Type
  • Primary Antibody
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Reactivity
  • Mouse
Shipping Ice Pack
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

Product Overview

Research Use Only

Minimum Cross reactivity to Human Serum Proteins

Validated Applications

Application data not specified. Contact us for validation guidance.

Key Specifications

Species Reactivity Mouse
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Catalog No. SA024

Immunogen / Antigen

Mouse IgG whole molecule

Reactivity Notes

This antibody has been validated for reactivity against Mouse targets. Cross-reactivity with other species may exist but has not been formally established.

Additional Information

Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free

Storage & Handling

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

Safety

For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.

What applications has the Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Rabbit] been validated for?

This antibody has been validated for: please consult the datasheet. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.

What is the recommended dilution range?

This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Which species does this antibody recognize?

This antibody has been confirmed reactive against: Mouse. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.

What is the immunogen / antigen used to generate this antibody?

Mouse IgG whole molecule

How should this antibody be stored and handled?

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.

Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today