| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ALOX5AP was used as the immunogen for the ALOX5AP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ALOX5AP Antibody / Arachidonate 5-lipoxygenase-activating protein is a anti-ALOX5AP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human. Reported localization: ER, nuclear membrane.
Key elements and design rationale
- Target: ALOX5AP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS
Biological background
Functionally, ALOX5AP antibody identifies a 161-amino-acid integral membrane protein located primarily in the nuclear envelope and endoplasmic reticulum. ALOX5AP functions as a scaffold that transfers arachidonic acid to ALOX5, catalyzing the first steps of leukotriene synthesis�conversion of arachidonic acid to leukotriene A4. This process produces potent signaling molecules that regulate vascular permeability, smooth muscle contraction, and leukocyte recruitment during inflammation.
The ALOX5AP gene is located on chromosome 13q12.3 and is highly expressed in leukocytes, macrophages, and endothelial cells. Expression increases in response to pro-inflammatory stimuli such as cytokines, lipopolysaccharides, and oxidative stress, linking ALOX5AP to innate immunity and inflammatory signaling networks.
Pathologically, ALOX5AP has been strongly associated with chronic inflammatory diseases including asthma, atherosclerosis, and stroke. Genetic polymorphisms in ALOX5AP are linked to increased cardiovascular risk and altered leukotriene production. Overactivation contributes to endothelial dysfunction and plaque instability. Research using ALOX5AP antibody supports studies in lipid mediator biosynthesis, immune cell signaling, and vascular inflammation.
ALOX5AP antibody is validated for western blotting, immunohistochemistry, and ELISA to detect lipid metabolism-associated proteins.
Structurally, Arachidonate 5-lipoxygenase-activating protein is an integral membrane protein containing four transmembrane helices that form a hydrophobic pocket for arachidonic acid binding and transfer. It interacts directly with ALOX5 at the nuclear membrane, coordinating substrate channeling during leukotriene biosynthesis. This antibody enables precise examination of ALOX5AP's role in lipid mediator production and inflammatory regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
