| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TUBA1C was used as the immunogen for the Alpha Tubulin antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Alpha Tubulin Antibody / TUBA1C is a anti-TUBA1C Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: TUBA1C
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Functionally, Alpha Tubulin antibody recognizes a conserved 50 kDa cytoskeletal protein that pairs with beta-tubulin to form heterodimers, the basic building blocks of microtubules. These structures are critical for maintaining cell polarity, providing tracks for motor proteins like kinesin and dynein, and ensuring accurate chromosome segregation during mitosis. Tubulin polymerization is regulated by GTP binding and hydrolysis, and is sensitive to factors such as temperature, post-translational modifications, and microtubule-associated proteins (MAPs). The equilibrium between polymerized microtubules and soluble tubulin dimers governs many cellular functions, including vesicle transport and organelle positioning.
Alpha tubulin undergoes various modifications such as acetylation, tyrosination, detyrosination, and polyglutamylation, which influence microtubule stability and motor protein interactions. Acetylated alpha tubulin is often used as a marker for stable microtubules, particularly in cilia and flagella. The Alpha Tubulin antibody is therefore widely applied as a loading control in western blotting, an internal cytoskeletal marker in immunofluorescence, and a reference protein for quantifying microtubule organization.
The TUBA gene family encodes several alpha-tubulin isoforms with tissue-specific expression patterns. TUBA1A is the predominant neuronal isoform and plays a major role in axon guidance and brain development. Mutations in TUBA1A have been associated with lissencephaly, cortical dysplasia, and microcephaly. Non-neuronal alpha-tubulin isoforms are equally essential for mitotic spindle integrity and intracellular transport in proliferating cells. Disruption of alpha-tubulin function, through chemical inhibitors such as colchicine or vinblastine, arrests cells in metaphase by depolymerizing microtubules.
Alpha Tubulin antibody provides a robust marker for assessing cytoskeletal architecture, cellular morphology, and proliferation. It is frequently used in combination with antibodies against beta tubulin, gamma tubulin, or actin to visualize cytoskeletal dynamics. In research involving neurobiology, alpha tubulin serves as a key indicator of axonal growth and neuronal polarity. In cancer studies, changes in tubulin expression and post-translational modification patterns reflect cytoskeletal reorganization during transformation, invasion, and drug resistance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
