| Field | Specification |
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| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ANXA1 was used as the immunogen for the Annexin A1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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Overview
Annexin A1 Antibody / Lipocortin-1 / ANXA1 is a anti-A1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, nucleus, cell membrane.
Key elements and design rationale
- Target: A1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS
Biological background
Annexin A1 is expressed in many tissues, especially in neutrophils, macrophages, epithelial cells, and the pituitary gland. Upon glucocorticoid stimulation, it translocates to the plasma membrane and is secreted into the extracellular space, where it binds formyl peptide receptor 2 (FPR2/ALX) on immune cells to inhibit migration and cytokine production. The protein also promotes efferocytosis and tissue repair during inflammation resolution. Dysregulation of Annexin A1 contributes to chronic inflammatory diseases, sepsis, and cancer progression.
The Annexin A1 antibody is widely used in immunology, endocrinology, and cancer research to study anti-inflammatory signaling, glucocorticoid response, and cell migration. Western blot analysis typically identifies a 37 kilodalton band corresponding to Annexin A1, while immunohistochemistry reveals cytoplasmic and membrane localization in activated leukocytes and epithelial cells. The antibody is also useful in identifying corticosteroid-mediated gene induction and verifying downstream targets of FPR2 signaling.
In cancer biology, Annexin A1 can act as a context-dependent modulator of tumor invasion and metastasis. It regulates cytoskeletal remodeling, apoptosis, and vesicle trafficking, influencing tumor cell motility. The Annexin A1 antibody helps delineate these pathways by enabling accurate detection in tumor and immune microenvironment studies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
