| Field | Specification |
|---|---|
| Target | |
| Alternative names | 14-3-3 protein sigma;Epithelial cell marker protein 1;Stratifin;SFN;HME1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human 14-3-3 sigma |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HBB-19 is a rabbit monoclonal antibody that recognizes 14-3-3 protein sigma (gene SFN) in human and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 27.8 kDa, and the supplier reports an observed band at about 28 kDa.
14-3-3 protein sigma (SFN, also called stratifin) is an epithelial member of the 14-3-3 family, adaptor proteins that bind phosphoserine or phosphothreonine motifs on many partners. Induced by p53 after DNA damage, it holds cells at the G2/M checkpoint and is frequently silenced in carcinomas, so it is studied in cell cycle control and cancer.
| Target | 14-3-3 protein sigma (gene SFN; UniProt P31947, human) |
|---|---|
| Clone | HBB-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 27.8 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human 14–3–3 sigma.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt describes 14-3-3 protein sigma as secreted and also located in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
