| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 zeta/delta, which shares 97.8% amino acid (aa) sequence identity with both mouse and rat 14-3-3 zeta/delta. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of YWHAZ (Alpha-galactosidase A) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® catalog # A01141-1. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 zeta/delta, which shares 97.8% amino acid (aa) sequence identity with both mouse and rat 14-3-3 zeta/delta.
- Molecular weight context: reported MW: 28 kDa; calculated MW: 47643 MW
- Reactivity: Human,Mouse,Rat
- Applications: IF, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Alpha-galactosidase A; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta. 14-3-3 protein zeta/delta (14-3-3ζ) is a protein that in humans is encoded by the YWHAZ gene on chromosome 8. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. Functional note: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Reported localization: Cytoplasm. Expression/tissue context: Widely expressed. Expressed at the highest levels in pancreas, heart, brain, skeletal muscle and placenta. Expressed at lower levels in lung, liver and kidney.
Research relevance and current trends
- Adapters: Researchers commonly examine how YWHAZ (Alpha-galactosidase A) relates to this theme using model systems and orthogonal readouts.
- Alzheimer's Disease: Researchers commonly examine how YWHAZ (Alpha-galactosidase A) relates to this theme using model systems and orthogonal readouts.
- Apoptosis: Researchers commonly examine how YWHAZ (Alpha-galactosidase A) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative YWHAZ (Alpha-galactosidase A) levels across conditions; band patterns may reflect isoforms and processing.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
