| Field | Specification |
|---|---|
| Target | |
| Alternative names | Natural killer cell receptor 2B4; NK cell type I receptor protein 2B4; NKR2B4; Non-MHC restricted killing associated; SLAM family; SLAMF4; Signaling lymphocytic activation molecule 4; CD244; Cd244; 2b4, Nmrk |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse 2B4/Cd244 recombinant protein (Position: Q20-N374). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets natural killer cell receptor 2B4 (gene Cd244) in mouse samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 44.8 kDa, and the supplier reports an observed band at about 65–75 kDa.
CD244, also known as 2B4, is a SLAM family receptor expressed on NK cells, some CD8+ T cells and myeloid cells. It binds CD48 and can deliver activating or inhibitory signals depending on the adaptor proteins present, so it is studied in NK-cell cytotoxicity and T-cell exhaustion.
| Target | Natural killer cell receptor 2B4 (gene Cd244; UniProt Q07763, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 44.8 kDa |
| Observed MW | 65–75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat spleen tissue, rat lung tissue, mouse spleen tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse 2B4/Cd244 fragment (Gln20–Asn374), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places natural killer cell receptor 2B4 in the membrane, cell membrane and membrane raft. Tissue expression noted by UniProt (mouse): Expressed in natural killer (NK) cells, T cells and dendritic cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
