| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Trophoblast glycoprotein;5T4 oncofetal antigen;5T4 oncofetal trophoblast glycoprotein;5T4 oncotrophoblast glycoprotein;M6P1;Wnt-activated inhibitory factor 1;WAIF1;TPBG;5T4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human 5T4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-5T4 TPBG Rabbit Monoclonal Antibody is an antibody targeting TPBG. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: EHE-20; isotype: Rabbit IgG; reactivity: Human; observed MW: 47 kDa; calculated MW: 46032 MW.
Boster Bio Anti-5T4 TPBG Rabbit Monoclonal Antibody catalog # M07442. Tested in WB, IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: TPBG — Trophoblast glycoprotein
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: EHE-20; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 47 kDa; Calculated: 46032 MW
Biological background
Protein function (datasheet): May function as an inhibitor of Wnt/beta-catenin signaling by inly interacting with LRP6 and blocking Wnt3a- dependent LRP6 internalization. .
Cellular localization (datasheet): Cell membrane ; Single-pass type I membrane protein .
Tissue details (datasheet): Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Developmental Biology,Lineage Markers,Lineage Specification,Stem Cells,Tumor Immunology,Tumor-Associated Antigens.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
