| Field | Specification |
|---|---|
| Target | |
| Alternative names | 40S ribosomal protein SA ;37 kDa laminin receptor precursor ;37LRP ;37/67 kDa laminin receptor ;LRP/LR ;67 kDa laminin receptor ;67LR ;Colon carcinoma laminin-binding protein;Laminin receptor 1 ;LamR ;Laminin-binding protein precursor p40 ;LBP/p40 ;Multidrug resistance-associated protein MGr1-Ag;NEM/1CHD4;RPSA ;LAMBR, LAMR1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human RPSA recombinant protein (Position: S2-S138). Human RPSA shares 100% and 99.3% amino acid (aa) sequence identity with mouse and rat RPSA, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets small ribosomal subunit protein uS2 (gene RPSA) in human samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 32.9 kDa, and the supplier reports an observed band at about 38–40 kDa.
RPSA (uS2) is a small-subunit ribosomal protein required for 40S assembly and late processing of 18S rRNA. It is also found at the cell surface, where it is known as the 37/67 kDa laminin receptor and mediates adhesion to the basement membrane; this form has also been studied as a receptor for prions and some viruses.
| Target | Small ribosomal subunit protein uS2 (gene RPSA; UniProt P08865, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 32.9 kDa |
| Observed MW | 38–40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human K562 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human RPSA fragment (Ser2–Ser138), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 100% and 99.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places small ribosomal subunit protein uS2 in the cell membrane, cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jianyi Zhang et al. (2023) Cytotoxicity of alkaline serine protease (ASPNJ) on Jurkat cells and its correlation with changes in the expression of membrane-associated proteins. Journal of Biochemical and Molecular Toxicology. 10.1002/jbt.23456
