| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alpha-2-macroglobulin;Alpha-2-M;C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5;A2M;CPAMD5;FWP007; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human A2M |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal Antibody is an antibody targeting A2M. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: ABAO-1; isotype: Rabbit IgG; reactivity: Human; observed MW: 60 kDa; calculated MW: 163291 MW.
Boster Bio Anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal Antibody catalog # M01498. Tested in WB, IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: A2M — Alpha-2-macroglobulin
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: ABAO-1; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 60 kDa; Calculated: 163291 MW
Biological background
Protein function (datasheet): Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Cellular localization (datasheet): Secreted .
Tissue details (datasheet): Secreted in plasma. .
Research relevance and current trends
- Commonly studied in contexts related to Cell Biology,Complement,Immunology,Innate Immunity,Protease Inhibitors,Proteolysis/Ubiquitin.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
