| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA-3-methyladenine glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; ADPG; N-methylpurine-DNA glycosylase; MPG; AAG; ANPG; MID1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human AAG/MPG recombinant protein (Position: Q20-A298). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes DNA-3-methyladenine glycosylase (gene MPG) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 32.9 kDa, and the supplier reports an observed band at about 37 kDa.
N-methylpurine DNA glycosylase (MPG, also called AAG) is a base excision repair enzyme that removes damaged bases produced by alkylating agents, such as 3-methyladenine and 7-methylguanine, as well as some deaminated purines. Its action starts repair of alkylation damage in DNA.
| Target | DNA-3-methyladenine glycosylase (gene MPG; UniProt P29372, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 32.9 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL/ml (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL/ml (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human Jurkat cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human AAG/MPG fragment (Gln20–Ala298), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA-3-methyladenine glycosylase in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
