| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-C motif chemokine 18;Alternative macrophage activation-associated CC chemokine 1;AMAC-1;CC chemokine PARC;Dendritic cell chemokine 1;DC-CK1;Macrophage inflammatory protein 4;MIP-4;Pulmonary and activation-regulated chemokine;Small-inducible cytokine A18;CCL18 (1-68);CCL18 (3-69);CCL18 (4-69);CCL18;AMAC1, DCCK1, MIP4, PARC, SCYA18; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human AAMP recombinant protein (Position: E235-R434). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of AAMP (Angio-associated migratory cell protein) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-AAMP CCL18 Antibody Picoband® catalog # PB9123. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human AAMP recombinant protein (Position: E235-R434). (reported region: E235-R434).
- Molecular weight context: reported MW: 52 kDa; calculated MW: 47 kDa
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Angio-associated migratory cell protein; C-C motif chemokine 18. AAMP, also known as Angio-associated, migratory cell protein, is a protein which in humans is encoded by the AAMP gene. It is mapped to 2q35. The gene product of AAMP is an immunoglobulin-type protein, which is found to be expressed strongly in endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics. It has been demonstrated that an AAMP peptide containing the putative heparan sulfate-binding domain binds to heparin and mediates heparin-sensitive cell adhesion. AAMP plays a role in angiogenesis and cell migration. In smooth muscle cell migration, it may act through the RhoA pathway. Functional note: Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. . Reported localization: Secreted. Expression/tissue context: Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid. .
Research relevance and current trends
- Cancer: Researchers commonly examine how AAMP (Angio-associated migratory cell protein) relates to this theme using model systems and orthogonal readouts.
- Chemokines: Researchers commonly examine how AAMP (Angio-associated migratory cell protein) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how AAMP (Angio-associated migratory cell protein) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative AAMP (Angio-associated migratory cell protein) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of AAMP (Angio-associated migratory cell protein) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Contains 8 WD repeats.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
