| Field | Specification |
|---|---|
| Target | |
| Alternative names | 4-aminobutyrate aminotransferase, mitochondrial;2.6.1.19; (S)-3-amino-2-methylpropionate transaminase;2.6.1.22;GABA aminotransferase;GABA-AT;Gamma-amino-N-butyrate transaminase;GABA transaminase;GABA-T;L-AIBAT;ABAT;GABAT; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human ABAT recombinant protein (Position: K388-K500). Human ABAT shares 93.9% and 94.5% amino acid (aa) sequence identity with mouse and rat ABAT, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects 4-aminobutyrate aminotransferase, mitochondrial (gene ABAT) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 56.4 kDa, and the supplier reports an observed band at about 54 kDa.
4-Aminobutyrate aminotransferase (ABAT, GABA-T) is a mitochondrial enzyme that breaks down the inhibitory neurotransmitter GABA to succinate semialdehyde. By controlling GABA levels in the brain it is an important target in neuroscience, and it is inhibited by the antiepileptic drug vigabatrin.
| Target | 4-aminobutyrate aminotransferase, mitochondrial (gene ABAT; UniProt P80404, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 56.4 kDa |
| Observed MW | 54 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HepG2 cells, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Recombinant human ABAT fragment (Lys388–Lys500), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.9% and 94.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 4-aminobutyrate aminotransferase, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Liver > pancreas > brain > kidney > heart > placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
