| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-binding cassette sub-family B member 10, mitochondrial;ATP-binding cassette transporter 10;ABC transporter 10 protein;Mitochondrial ATP-binding cassette 2;M-ABC2;ABCB10; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB10, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ATP-binding cassette sub-family B member 10, mitochondrial (gene ABCB10) in human and rat samples and is validated for Western blot. The predicted molecular weight is 79.1 kDa; the supplier reports observed bands at 79 kDa, 65 kDa.
ABCB10 is an ATP-binding cassette transporter in the inner mitochondrial membrane. It exports biliverdin from mitochondria and supports early heme synthesis in developing red cells, partly by stabilizing the iron importer mitoferrin-1, so it is studied in hemoglobin production and oxidative stress.
| Target | ATP-binding cassette sub-family B member 10, mitochondrial (gene ABCB10; UniProt Q9NRK6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 79.1 kDa |
| Observed MW | 79 kDa, 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat cardiac muscle tissue, COLO320 cells, 22RV1 cells, PANC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human ABCB10. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places ATP-binding cassette sub-family B member 10, mitochondrial in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
