| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bile salt export pump;ATP-binding cassette sub-family B member 11;ABCB11;BSEP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB11, different from the related mouse and rat sequences by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes bile salt export pump (gene ABCB11) in human, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 146.4 kDa, and the supplier reports an observed band at about 146 kDa.
The bile salt export pump (ABCB11, BSEP) moves conjugated bile salts from hepatocytes into bile canaliculi using ATP. It is the main driver of bile salt-dependent bile flow, and its loss or inhibition by drugs causes cholestasis, making it important in liver physiology and drug safety studies.
| Target | Bile salt export pump (gene ABCB11; UniProt O95342, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 146.4 kDa |
| Observed MW | 146 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Mouse) |
Samples with a confirmed band (WB): human hcct tissue, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse liver tissue, rat liver tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): mouse liver tissue.
Immunogen
Synthetic peptide from the C-terminal region of human ABCB11. It differs from the mouse and rat sequences at three residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places bile salt export pump in the apical cell membrane, recycling endosome membrane, endosome and cell membrane. Tissue expression noted by UniProt (human): Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tao Jiang et al. (2025) Achyranthes bidentata polysaccharides alleviate alcohol-induced gut-liver injury via Farnesoid X Receptor pathway. Food Bioscience. 10.1016/j.fbio.2025.106748
Zhihui Li et al. (2025) Emodin and physcion alleviate cholestatic liver injury by targeting FXR: hepatoprotective components identified in processed Polygonum multiflorum Thunb. using a comprehensive two-dimensional biochromatography system. Frontiers in Pharmacology. 10.3389/fphar.2025.1706401
Baoshen He et al. (2021) Hypercholesterolemia risk associated Abca6 does not regulate lipoprotein metabolism in mice or hamster. Biochimica et Biophysica ACTA-Molecular and Cell Biology of Lipids. 10.1016/j.bbalip.2021.159006
Masahiko Itoh et al. (2020) The zonula occludens protein family regulates the hepatic barrier system in the murine liver. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2020.165994
Jian Ding et al. (2024) Loss of hepatocyte Usp53 protects mice from a form of xenobiotic-induced liver injury. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2024.167624
Antonia Felzen et al. (2025) Generation and characterization of a mouse model for bile salt export pump deficiency with the p.E297G mutation. Biochimica et Biophysica ACTA-Molecular and Cell Biology of Lipids. 10.1016/j.bbalip.2025.159697
Kholoud Alaa Elmihi et al. (2025) Female ethanolamine phosphate phospholyase knockout mice resisted high-fat diet-induced obesity with attenuated hepatic cholesterol deposition. American Journal of Physiology-Gastrointestinal and Liver Physiology. 10.1152/ajpgi.00386.2024
Zhinan Jin et al. (2025) Geniposide induces hepatotoxicity via the bile acid-induced activation of NLRP3 inflammasome and regulation of the FXR/PERK/TXNIP pathway. Toxicology Letters. 10.1016/j.toxlet.2025.111740
Hui-Yu She et al. (2025) A liver-specific mouse model for MYO5B-associated cholestasis reveals a toxic gain-of-function as underlying disease mechanism. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2025.151669
Masahiko Itoh et al. (2024) Molecular alterations associated with pathophysiology in liver-specific ZO-1 and ZO-2 knockout mice. Cell Structure and Function. 10.1247/csf.24046
