| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-binding cassette sub-family C member 3; Canalicular multispecific organic anion transporter 2; Multi-specific organic anion transporter D; MOAT-D; Multidrug resistance-associated protein 3; ABCC3; CMOAT2; MLP2; MRP3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ABCC3 recombinant protein (Position: H22-G1351). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes ATP-binding cassette sub-family C member 3 (gene ABCC3) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 169.3 kDa, and the supplier reports an observed band at about 200 kDa.
MRP3 (ABCC3) is an ATP-dependent transporter on the basolateral membranes of hepatocytes and intestinal cells. It moves conjugated metabolites and bile acids from these cells into the blood, providing an alternative route when biliary excretion is impaired, and it is studied in drug metabolism and cholestasis.
| Target | ATP-binding cassette sub-family C member 3 (gene ABCC3; UniProt O15438, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 169.3 kDa |
| Observed MW | 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human ABCC3 fragment (His22–Gly1351), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ATP-binding cassette sub-family C member 3 in the basolateral cell membrane and basal cell membrane. Tissue expression noted by UniProt (human): Mainly expressed in the liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
