| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-binding cassette sub-family E member 1;2'-5'-oligoadenylate-binding protein;HuHP68;RNase L inhibitor;Ribonuclease 4 inhibitor;RNS4I;ABCE1;RLI, RNASEL1, RNASELI, RNS4I;OK/SW-cl.40; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human ABCE1 recombinant protein (Position: K419-D599). Human ABCE1 shares 100% amino acid (aa) sequence identity with mouse ABCE1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ATP-binding cassette sub-family E member 1 (gene ABCE1) in human, mouse and rat samples and is validated for flow cytometry, immunoprecipitation, ICC/IF and Western blot. The predicted molecular weight is 67.3 kDa, and the supplier reports an observed band at about 67 kDa.
ABCE1 is a highly conserved ATPase that recycles ribosomes by splitting them into large and small subunits after translation ends or when ribosomes stall. It also takes part in translation initiation and is essential for cell viability, so it is studied in protein synthesis and quality control.
| Target | ATP-binding cassette sub-family E member 1 (gene ABCE1; UniProt P61221, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 67.3 kDa |
| Observed MW | 67 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HepG2 cells lysate, human A431 cells lysate, human U2OS cells lysate, human Jurkat cells lysate, rat brain tissue lysates, mouse brian tissue lysates, mouse 4T1 cells lysate, mouse NIH/3T3 cells lysate.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed result (IP): A431 cells.
Samples with a confirmed signal (flow cytometry): U251 cells.
Immunogen
Recombinant human ABCE1 fragment (Lys419–Asp599), expressed in E. coli. Sequence identity with the mouse ortholog: 100%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ATP-binding cassette sub-family E member 1 in the cytoplasm and mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
