Anti-ABCE1 Antibody Picoband®

Overview

This antibody is intended for detection of ABCE1 (ATP-binding cassette sub-family E member 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-ABCE1 Antibody Picoband® catalog # PB10020. Tested in Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: E. coli-derived human ABCE1 recombinant protein (Position: K419-D599). Human ABCE1 shares 100% amino acid (aa) sequence identity with mouse ABCE1. (reported region: K419-D599).
• Molecular weight context: reported MW: 67 kDa; calculated MW: 67314 MW
• Reactivity: Human,Mouse,Rat
• Applications: Flow Cytometry, IP, IF, ICC, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

ATP-binding cassette sub-family E member 1; ATP-binding cassette sub-family E member 1. ATP binding cassette E1 (ABCE1, also RNase L inhibitor) is an ATPase found in humans involved in viral assembly. It is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily. ABCE1 inhibits the action of ribonuclease L. Ribonuclease L normally binds to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) and inhibits the interferon-regulated 2-5A/RNase L pathway, which is used by viruses. ABCE1 heterodimerize with ribonuclease L and prevents its interaction with 2-5A, antagonizing the anti-viral properties of ribonuclease L, and allow the virus to synthesize viral proteins. It has also been implicated to have an effect in tumorcell proliferation and antiapoptosis. Functional note: Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. . Reported localization: Cytoplasm . Mitochondrion . Localized to clusters of virus formation at the plasma membrane. Expression/tissue context: Liver > pancreas > brain > kidney > heart > placenta.

Research relevance and current trends

• Adapters: Researchers commonly examine how ABCE1 (ATP-binding cassette sub-family E member 1) relates to this theme using model systems and orthogonal readouts.
• Antiviral Signaling: Researchers commonly examine how ABCE1 (ATP-binding cassette sub-family E member 1) relates to this theme using model systems and orthogonal readouts.
• ATPases: Researchers commonly examine how ABCE1 (ATP-binding cassette sub-family E member 1) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative ABCE1 (ATP-binding cassette sub-family E member 1) levels across conditions; band patterns may reflect isoforms and processing.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
• Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Family / similarity context: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.