| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ATP-binding cassette sub-family G member 2;Breast cancer resistance protein;CDw338;Mitoxantrone resistance-associated protein;Placenta-specific ATP-binding cassette transporter;Urate exporter;CD338;ABCG2;ABCP, BCRP, BCRP1, MXR; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human ABCG2 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-ABCG2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AODG-1; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-ABCG2/Bcrp Rabbit Monoclonal Antibody catalog # M00457. Tested in WB application. This antibody reacts with Human.
Key elements and design rationale
- Target: ABCG2 (ATP-binding cassette sub-family G member 2).
- Antibody format: Monoclonal; clone AODG-1; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
ABCG2 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. Implicated in the efflux of numerous drugs and xenobiotics: mitoxantrone, the photosensitizer pheophorbide, camptothecin, methotrexate, azidothymidine (AZT), and the anthracyclines daunorubicin and doxorubicin. . Reported cellular localization context: Cell membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Tissue expression notes (as provided): Highly expressed in placenta. Low expression in small intestine, liver and colon. .
Research relevance and current trends
- Research context keywords from the source record include: Signal Transduction,Stem Cells,Surface Molecules,Tags & Cell Markers,Tumor Associated.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate ABCG2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ABCG2 expression by Western blot in cell or tissue lysates, Compare relative ABCG2 levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 73 kDa; calculated MW: 72314 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 73 kDa
- Cellular localization (provided): Cell membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein.
- Tissue details (provided): Highly expressed in placenta. Low expression in small intestine, liver and colon. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
