| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ATP-binding cassette sub-family G member 4;ABCG4;WHITE2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ABCG4, different from the related mouse and rat sequences by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-ABCG4 Antibody Picoband® is an antibody targeting ABCG4. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Rat,Mouse,Human; observed MW: 280 kDa; calculated MW: 71896 MW.
Boster Bio Anti-ABCG4 Antibody catalog # PA2067. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: ABCG4 — ATP-binding cassette sub-family G member 4
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Rat,Mouse,Human
- Molecular weight guidance: Observed: 280 kDa; Calculated: 71896 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): May be involved in macrophage lipid homeostasis.
Scientific background (datasheet): ABCG4 (ATP-Binding Cassette, Subfamily G, Member 4), is a protein that in humans is encoded by the ABCG4 gene. The protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. This protein is a member of the White subfamily and is expressed predominantly in liver tissue. By genomic sequence analysis, Engel et al. (2001) mapped the ABCG4 gene to chromosome 11q23.3. Engel et al. (2001) demonstrated 5-fold induction of ABCG4 following treatment of normal monocyte-derived macrophages with the LXR and RXR agonists 9-cis retinoic acid and 22R hydroxycholesterol. Removal of cholesterol from macrophages by cyclodextrin decreased ABCG4 message levels.
Cellular localization (datasheet): Membrane ; Multi-pass membrane protein .
Tissue details (datasheet): Highly expressed in brain tissues with the exception of the spinal cord. .
Sequence similarities (datasheet): Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cardiovascular,Cell Type Marker,Cholesterol Metabolism,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Neuron Marker,Neuroscience,Pathways and Processes,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
