| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Amiloride-sensitive amine oxidase [copper-containing];DAO;Diamine oxidase;1.4.3.22 ;Amiloride-binding protein 1;Amine oxidase copper domain-containing protein 1;Histaminase;Kidney amine oxidase;KAO;AOC1;ABP1, DAO1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ABP1, different from the related mouse sequence by ten amino acids, and from the related rat sequence by eight amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of AOC1 (Amiloride-sensitive amine oxidase [copper-containing]) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-ABP1/AOC1 Antibody Picoband® catalog # PB10040. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human ABP1, different from the related mouse sequence by ten amino acids, and from the related rat sequence by eight amino acids.
- Molecular weight context: reported MW: 85 kDa; calculated MW: 85378 MW
- Reactivity: Human,Monkey
- Applications: IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Amiloride-sensitive amine oxidase [copper-containing]; Amiloride-sensitive amine oxidase [copper-containing]. This gene encodes a metal-binding membrane glycoprotein that oxidatively deaminates putrescine, histamine, and related compounds. The encoded protein is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. Functional note: Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. Reported localization: Secreted, extracellular space. Expression/tissue context: Placenta and kidney.
Research relevance and current trends
- Mechanistic pathway studies: Researchers commonly examine how AOC1 (Amiloride-sensitive amine oxidase [copper-containing]) relates to this theme using model systems and orthogonal readouts.
- Biomarker profiling across models: Researchers commonly examine how AOC1 (Amiloride-sensitive amine oxidase [copper-containing]) relates to this theme using model systems and orthogonal readouts.
- Perturbation-response experiments (time-course/dose–response): Researchers commonly examine how AOC1 (Amiloride-sensitive amine oxidase [copper-containing]) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative AOC1 (Amiloride-sensitive amine oxidase [copper-containing]) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of AOC1 (Amiloride-sensitive amine oxidase [copper-containing]) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
