| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Acid-sensing ion channel 2;ASIC2;Amiloride-sensitive brain sodium channel;Amiloride-sensitive cation channel 1, neuronal;Amiloride-sensitive cation channel neuronal 1;Brain sodium channel 1;BNC1;BNaC1;Mammalian degenerin homolog;ASIC2;ACCN, ACCN1, BNAC1, MDEG; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ACCN1, different from the related mouse and rat sequences by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ASIC2 (Acid-sensing ion channel 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-ACCN1/ASIC2 Antibody Picoband® catalog # PB10046. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human ACCN1, different from the related mouse and rat sequences by one amino acid.
- Molecular weight context: reported MW: 65 kDa; calculated MW: 57709 MW
- Reactivity: Human,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Acid-sensing ion channel 2; Acid-sensing ion channel 2. Amiloride-sensitive cation channel 1, neuronal, also known as ASIC2, is a protein that in humans is encoded by the ACCN1 gene. This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified. Functional note: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li (+) and K (+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate. Reported localization: Cell membrane ; Multi-pass membrane protein . Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes. . Expression/tissue context: Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary. .
Research relevance and current trends
- Neuroscience: Researchers commonly examine how ASIC2 (Acid-sensing ion channel 2) relates to this theme using model systems and orthogonal readouts.
- Neurotransmission: Researchers commonly examine how ASIC2 (Acid-sensing ion channel 2) relates to this theme using model systems and orthogonal readouts.
- Receptors / Channels: Researchers commonly examine how ASIC2 (Acid-sensing ion channel 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ASIC2 (Acid-sensing ion channel 2) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
