| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lysosomal acid phosphatase; LAP; Tartrate-inhibitable acid phosphatase; ACP2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ACP2, which shares 100% and 96.4% amino acid (aa) sequence identity with mouse and rat ACP2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets lysosomal acid phosphatase (gene ACP2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 48.3 kDa, and the supplier reports an observed band at about 76 kDa.
Lysosomal acid phosphatase (ACP2) removes phosphate groups from substrates under the acidic conditions of the lysosome. It is thought to help remove mannose-6-phosphate from lysosomal enzymes after delivery, and it is studied in lysosome function.
| Target | Lysosomal acid phosphatase (gene ACP2; UniProt P11117, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 48.3 kDa |
| Observed MW | 76 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human T-47D cells, human HeLa cells, human U251 cells, human RT4 cells, rat liver tissue, rat pancreas tissue, mouse liver tissue, mouse pancreas tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human ACP2. Sequence identity with the mouse and rat orthologs: 100% and 96.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places lysosomal acid phosphatase in the lysosome membrane and lysosome lumen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
