| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pro-opiomelanocortin;POMC;Corticotropin-lipotropin;NPP;Melanotropin gamma;Gamma-MSH;Potential peptide;Corticotropin;Adrenocorticotropic hormone;ACTH;Melanotropin alpha;Alpha-MSH;Corticotropin-like intermediary peptide;CLIP;Lipotropin beta;Beta-LPH;Lipotropin gamma;Gamma-LPH;Melanotropin beta;Beta-MSH;Beta-endorphin;Met-enkephalin;POMC; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ACTH, different from the related mouse and rat sequences by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes pro-opiomelanocortin (gene POMC) in human, mouse and rat samples and is validated for IHC. The predicted molecular weight is 29.4 kDa.
Pro-opiomelanocortin (POMC) is a precursor protein cut into several active peptides, including ACTH, α-, β- and γ-MSH and β-endorphin. These act on melanocortin and opioid receptors to regulate cortisol release, skin pigmentation, appetite and energy balance. POMC is a key marker of pituitary corticotrophs and hypothalamic appetite-regulating neurons.
| Target | Pro-opiomelanocortin (gene POMC; UniProt P01189, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 29.4 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat, Human) |
|---|
Samples with confirmed staining (IHC): mouse kidney tissue, rat brain tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the middle region of human ACTH. It differs from the mouse and rat sequences at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes pro-opiomelanocortin as a secreted protein. Tissue expression noted by UniProt (human): ACTH and MSH are produced by the pituitary gland.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
