| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Actin, alpha skeletal muscle;Alpha-actin-1;ACTA1;ACTA; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe,attached to a Multiple Antigen Peptide (MAP) backbone. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Actin ACTA1 Antibody (Monoclonal, AC-40) is an antibody targeting Actin. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: AC-40; isotype: Mouse IgG2a; reactivity: Chicken,Human,Mouse,Rat; observed MW: 43 kDa; calculated MW: 42051 MW.
Boster Bio Anti-Actin ACTA1 Antibody (Monoclonal, AC-40) catalog # MA1000. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Key elements and design rationale
- Target: Actin — Actin, alpha skeletal muscle
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: AC-40; Isotype: Mouse IgG2a
- Species reactivity: Chicken,Human,Mouse,Rat
- Molecular weight guidance: Observed: 43 kDa; Calculated: 42051 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Scientific background (datasheet): Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
Cellular localization (datasheet): Cytoplasm, cytoskeleton.
Tissue details (datasheet): Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Research relevance and current trends
- Commonly studied in contexts related to Actin,Actin Assembly,Actin Binding Proteins,Actin, etc.,Apoptosis,Cancer,Cell Death,Cytoskeleton,Cytoskeleton/ECM,Invasion/Microenvironment,Microfilaments,Receptors,Signal Transduction,Subcellular Markers,Tags & Cell Markers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
