| Field | Specification |
|---|---|
| Target | |
| Alternative names | Adenosine deaminase |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively. |
| Molecular weight | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 6D4 is a mouse monoclonal antibody that recognizes adenosine deaminase (gene ADA) in human samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 40.8 kDa, and the supplier reports an observed band at about 45 kDa.
Adenosine deaminase (ADA) converts adenosine and deoxyadenosine to inosine and deoxyinosine, controlling adenosine levels inside and outside cells. It is essential for lymphocyte development, and ADA deficiency causes a form of severe combined immunodeficiency (SCID).
| Target | Adenosine deaminase (gene ADA; UniProt P00813, human) |
|---|---|
| Clone | 6D4 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 40.8 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human HeLa cells, human placenta tissue, human A549 cells, human MCF-7 cells, human U-937 cells, human U2OS cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human rectal cancer tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human ADA fragment (Gln135–Leu363), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 82.5% and 82.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places adenosine deaminase in the cell membrane, cell junction and cytoplasmic vesicle lumen. Tissue expression noted by UniProt (human): Found in all tissues, occurs in large amounts in T-lymphocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Jing et al. (2026) Oncogenic DCTPP1/MYC feedback loop rewires pyrimidine metabolism to drive hepatocellular carcinoma. Functional & Integrative Genomics. 10.1007/s10142-026-01870-8
