| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Aquaporin-1;AQP-1;Aquaporin-CHIP;Urine water channel;Water channel protein for red blood cells and kidney proximal tubule;AQP1;CHIP28; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human ADA |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-ADA antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23A88; isotype IgG; reactivity: Human,Rat. Reported application contexts include WB, ICC, IF, IP (as provided in the source record). Boster Bio Anti-ADA Rabbit Monoclonal Antibody catalog # M00866-1. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: ADA (Aquaporin-1).
- Antibody format: Monoclonal; clone 23A88; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
ADA (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the ion of an osmotic gradient. . Reported cellular localization context: Cell membrane ; Multi-pass membrane protein . Tissue expression notes (as provided): Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. .
Research relevance and current trends
- Research context keywords from the source record include: Channels,Metabolism,Plasma Membrane,Signal Transduction,Vitamins/Minerals.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate ADA antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ADA expression by Western blot in cell or tissue lysates, Localize ADA by immunofluorescence/immunocytochemistry in cultured cells, Enrich ADA by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 45 kDa; calculated MW: 28526 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 45 kDa
- Cellular localization (provided): Cell membrane ; Multi-pass membrane protein .
- Tissue details (provided): Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
