| Field | Specification |
|---|---|
| Target | |
| Alternative names | A disintegrin and metalloproteinase with thrombospondin motifs 9; ADAM-TS 9; ADAM-TS9; ADAMTS-9; ADAMTS9; KIAA1312 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ADAMTS9 recombinant protein (Position: F288-H1853). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects A disintegrin and metalloproteinase with thrombospondin motifs 9 (gene ADAMTS9) in human and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 216.5 kDa, and the supplier reports an observed band at about 216 kDa.
ADAMTS9 is a secreted metalloprotease that cleaves the proteoglycans aggrecan and versican and helps remodel the extracellular matrix. It is needed for several developmental processes and is studied in connective tissue biology and cilia-related development.
| Target | A disintegrin and metalloproteinase with thrombospondin motifs 9 (gene ADAMTS9; UniProt Q9P2N4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 216.5 kDa |
| Observed MW | 216 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SH-SY5Y cells, rat H9C2(2–1) cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): THP-1 cells, U2OS cells.
Immunogen
Recombinant human ADAMTS9 fragment (Phe288–His1853), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places A disintegrin and metalloproteinase with thrombospondin motifs 9 in the endoplasmic reticulum, cell projection and recycling endosome. Tissue expression noted by UniProt (human): Highly expressed in all fetal tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
