Anti-Adiponectin/ADIPOQ Antibody Picoband®

SKU:BHA21007474
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Boster Bio
Boster Bio
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Overview
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Anti-ADIPOQ antibody (Rabbit, Polyclonal, Rabbit IgG) recommended for WB, IHC, Flow Cytometry, ELISA Commonly used in Oncology & Angiogenesis studies where these format selectors and application compatibility are required.
Target ADIPOQ
Host Rabbit
Reactivity Mouse,Rat
Isotype Rabbit IgG
Application(s) WB, IHC, Flow Cytometry, ELISA
Options selector
Catalog no. Size Conjugation
PB9011 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated; 100 ug/vial — Carrier Free
      Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
      Form: Lyophilized
      Applications: IHC,WB
      Application details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
      Western blot, 0.1-0.5μg/ml, Rat
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
    • 100 ug/vial — Biotin; 100 ug/vial — Cy3; 100 ug/vial — Fluoro488; 100 ug/vial — Fluoro550; 100 ug/vial — Fluoro594; 100 ug/vial — FITC; 100 ug/vial — APC; 100 ug/vial — PE; 100 ug/vial — Fluoro647
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
      Form: Liquid
      Applications: Flow Cytometry | WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cells
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
    • 100 ug/vial — HRP
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
      Form: Liquid
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No PB9011
Alternative Names 30 kDa adipocyte complement-related protein ;Adiponectin, C1Q and collagen domain containing ;Protein Adipoq ;Adipoq ;Acdc ;rCG_36676 ;
Clonality
  • Polyclonal
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Host Rabbit
Immunogen E.coli-derived rat Adiponectin recombinant protein (Position: I19-N244). Rat Adiponectin shares 90% amino acid (aa) sequence identity with mouse Adiponectin.
Isotype
  • Rabbit IgG
Molecular Weight 26 kDa
Product Type
  • Antibodies
  • Primary Antibodies
Reactivity
  • Mouse
  • Rat
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Target ADIPOQ
UniProt # Q8K3R4

Overview

Anti-Adiponectin/ADIPOQ Antibody Picoband® is an antibody targeting ADIPOQ. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Mouse,Rat; observed MW: 26 kDa; calculated MW: 26410 MW.

Boster Bio Anti-Adiponectin/ADIPOQ Antibody Picoband® catalog # PB9011. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

  • Target: ADIPOQ — adiponectin, C1Q and collagen domain containing
  • Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
  • Species reactivity: Mouse,Rat
  • Molecular weight guidance: Observed: 26 kDa; Calculated: 26410 MW

Specificity note: No cross reactivity with other proteins.

Biological background

Scientific background (datasheet): Adiponectin, also known as APM1 or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. It is mapped to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, it has been showed that Adiponectin is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, it has been found that links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. This gene is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.

Research relevance and current trends

  • Commonly studied in contexts related to Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Diabetes,Diabetes-associated,Growth Factors/Hormones,Heart Disease,Lipids/Lipoproteins,Mesenchymal Stem Cells,Metabolism,Metabolism Processes,Neurology Process,Neuroscience,Obesity,Pathways and Processes,Response To Hypoxia,Signal Transduction,Stem Cells.
  • Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
  • Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

  • Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
  • ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
  • Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
  • Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

  • Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
  • Cross-reactivity (datasheet): No cross-reactivity with other proteins
  • Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Will PB9011 anti-Adiponectin/ADIPOQ antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
You can see on the product datasheet, PB9011 anti-Adiponectin/ADIPOQ antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Would anti-Adiponectin/ADIPOQ antibody PB9011 work for WB with adipose tissue?
According to the expression profile of adipose tissue, ADIPOQ is highly expressed in adipose tissue. So, it is likely that anti-Adiponectin/ADIPOQ antibody PB9011 will work for WB with adipose tissue.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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