| Field | Specification |
|---|---|
| Target | |
| Alternative names | AFG3-like protein 2;3.4.24.-;Paraplegin-like protein;AFG3L2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human AFG3L2 recombinant protein (Position: R168-D250). Human AFG3L2 shares 100% amino acid (aa) sequence identity with mouse AFG3L2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mitochondrial inner membrane m-AAA protease component AFG3L2 in human, mouse and rat samples and is validated for flow cytometry, immunoprecipitation, ICC/IF and Western blot. The predicted molecular weight is 88.6 kDa, and the supplier reports an observed band at about 89 kDa.
AFG3L2 is the catalytic subunit of the m-AAA protease in the inner mitochondrial membrane. It combines ATPase and protease activities to unfold and degrade misfolded or damaged membrane proteins, maintaining mitochondrial protein quality, and it is important for neuronal and axonal development; mutations are linked to inherited ataxias.
| Target | Mitochondrial inner membrane m-AAA protease component AFG3L2 (gene AFG3L2; UniProt Q9Y4W6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 88.6 kDa |
| Observed MW | 89 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human Jurkat cells, human MDA-MB-453 cells, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed result (IP): MCF-7 cells.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human AFG3L2 fragment (Arg168–Asp250), expressed in E. coli. Sequence identity with the mouse ortholog: 100%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitochondrial inner membrane m-AAA protease component AFG3L2 in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
