| Field | Specification |
|---|---|
| Target | |
| Alternative names | Arf-GAP domain and FG repeat-containing protein 1;HIV-1 Rev-binding protein;Nucleoporin-like protein RIP;Rev-interacting protein;Rev/Rex activation domain-binding protein;AGFG1;HRB, RAB, RIP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human AGFG1, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
This rabbit polyclonal antibody detects arf-GAP domain and FG repeat-containing protein 1 (gene AGFG1) in human and rat samples and is validated for flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 58.3 kDa, and the supplier reports an observed band at about 58 kDa.
AGFG1 (also called HRB) is an Arf-GAP domain protein with FG repeats. It is needed for vesicle docking and fusion during acrosome formation in sperm, and during HIV-1 infection it acts as a cofactor for Rev, helping Rev-bound viral RNAs leave the nuclear periphery for the cytoplasm.
| Target | Arf-GAP domain and FG repeat-containing protein 1 (gene AGFG1; UniProt P52594, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 58.3 kDa |
| Observed MW | 58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human HeLa cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Synthetic peptide from the N-terminal region of human AGFG1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places arf-GAP domain and FG repeat-containing protein 1 in the nucleus and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Bing-Jie Hu et al. (2019) Sequence and time course of depletion of cardiac cellular proteins and accumulation of plasma proteins in rat early ischemic myocardium. Legal Medicine. 10.1016/j.legalmed.2019.04.001
