| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein argonaute-1;Argonaute1;hAgo1;Argonaute RISC catalytic component 1;Eukaryotic translation initiation factor 2C 1;eIF-2C 1;eIF2C 1;Putative RNA-binding protein Q99;AGO1;EIF2C1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human AGO1 recombinant protein (Position: E376-R409). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-AGO1 Antibody Picoband® is an antibody for AGO1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: AGO1 (Protein argonaute-1); UniProt: Q9UL18
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 97 kDa, calculated 97214 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-AGO1 Antibody Picoband® catalog # A00826-3.
Biological background
Biological context: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). .
Expression and localization notes: cellular localization: Cytoplasm, P-body ., tissue context: Detected in blood plasma (at protein level)..
Common research applications
- Western blotting (WB): Compare AGO1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of AGO1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify AGO1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Cytoplasm, P-body .
- Tissue details: Detected in blood plasma (at protein level).
- Research category: Cell Biology,Cell Cycle,Cell Division,Cytokinesis,DNA/RNA,Epigenetics and Nuclear Signaling,Nuclear Receptors,Nuclear Signaling Pathways,RNA Processing,Translation
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
