| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein argonaute-2 ;Argonaute2 ;hAgo2;3.1.26.n2 ;Argonaute RISC catalytic component 2;Eukaryotic translation initiation factor 2C 2 ;eIF-2C 2 ;eIF2C 2 ;PAZ Piwi domain protein;PPD;Protein slicer ;AGO2;EIF2C2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Ago2/ eIF2C2, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes protein argonaute-2 (gene AGO2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 97.2 kDa, and the supplier reports an observed band at about 97 kDa.
Argonaute-2 (AGO2) is the core of the RNA-induced silencing complex (RISC). Guided by a bound microRNA or siRNA, it finds complementary mRNAs and either cleaves them through its endonuclease (slicer) activity or represses their translation, making it central to RNA interference and microRNA research.
| Target | Protein argonaute-2 (gene AGO2; UniProt Q9UKV8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 97.2 kDa |
| Observed MW | 97 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human Jurkat cells, human 293T cells, human K562 cells, human MCF-7 cells, rat lung tissue, rat pancreas tissue, mouse pancreas tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human ago2/ eIF2C2. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein argonaute-2 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ming Guo et al. (2024) Vibrio splendidus infection promotes circRNA-FGL1-regulated coelomocyte apoptosis via competitive binding to Myc with the deubiquitinase OTUB1 in Apostichopus japonicus. PLoS Pathogens. 10.1371/journal.ppat.1012463
Xiaofang Cheng et al. (2020) MEG3 Promotes Differentiation of Porcine Satellite Cells by Sponging miR-423-5p to Relieve Inhibiting Effect on SRF. Cells. 10.3390/cells9020449
Li Meng et al. (2025) CircHOMER1 in mediating porcine skeletal muscle development. BMC Genomics. 10.1186/s12864-025-11991-2
Wang Kai et al. (2024) LncRNA S100PBP promotes proliferation and steroid hormone synthesis of granulosa cells by sponging MiR-2285bc-BMPR2 in bovine. Biology of Reproduction. 10.1093/biolre/ioae033
