| Field | Specification |
|---|---|
| Target | |
| Alternative names | Agouti-related protein; Agrp; Agrt; Art |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse AGRP recombinant protein (Position: S82-T131). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets agouti-related protein (gene Agrp) in human, mouse and rat samples and is validated for ELISA and IHC. The predicted molecular weight is 14.4 kDa.
Agouti-related protein (AGRP) is a neuropeptide made by hypothalamic neurons that blocks the melanocortin receptors MC3R and MC4R. By opposing α-MSH signaling it promotes feeding and reduces energy expenditure, so it is a key target in research on appetite, obesity and energy balance.
| Target | Agouti-related protein (gene Agrp; UniProt P56473, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 14.4 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): mouse lung tissue, rat lung tissue, rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant mouse AGRP fragment (Ser82–Thr131), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes agouti-related protein as secreted and also located in the Golgi apparatus lumen. Tissue expression noted by UniProt (mouse): Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhou Xihong et al. (2018) Long-Term l-Serine Administration Reduces Food Intake and Improves Oxidative Stress and Sirt1/NFκB Signaling in the Hypothalamus of Aging Mice. Frontiers in Endocrinology. 10.3389/fendo.2018.00476