Anti-AGRP Antibody

SKU:BHA21001403
Research Validated
—
Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-Agrp antibody (Rabbit polyclonal, Rabbit IgG). Validated for ELISA and immunohistochemistry. Reactivity: Human, Mouse, Rat. Recognizes agouti-related protein. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target Agrp
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) ELISA, IHC
Options selector
Catalog no. Size Conjugation
A00272-1 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: ELISA,IHC
      Application details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Agrp
Alternative names Agouti-related protein; Agrp; Agrt; Art
UniProt # P56473
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • ELISA
  • Immunohistochemistry
Immunogen E. coli-derived mouse AGRP recombinant protein (Position: S82-T131).
Molecular weight 14.4 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Secreted.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) A00272-1
Main SKU BHA21001403
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody targets agouti-related protein (gene Agrp) in human, mouse and rat samples and is validated for ELISA and IHC. The predicted molecular weight is 14.4 kDa.

Agouti-related protein (AGRP) is a neuropeptide made by hypothalamic neurons that blocks the melanocortin receptors MC3R and MC4R. By opposing α-MSH signaling it promotes feeding and reduces energy expenditure, so it is a key target in research on appetite, obesity and energy balance.

Target Agouti-related protein (gene Agrp; UniProt P56473, mouse)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3
Calculated MW 14.4 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 0.5–1 µg/mL
ELISA 0.1–0.5 µg/mL

Samples with confirmed staining (IHC): mouse lung tissue, rat lung tissue, rat testis tissue.

Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.

Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).

Immunogen

Recombinant mouse AGRP fragment (Ser82–Thr131), expressed in E. coli.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt describes agouti-related protein as secreted and also located in the Golgi apparatus lumen. Tissue expression noted by UniProt (mouse): Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: IHC (paraffin sections) 0.5–1 µg/mL; ELISA 0.1–0.5 µg/mL. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.Is a blocking reagent available for this antibody?
A.A matching blocking reagent for competition (specificity) controls can be supplied on request. Contact info@biohippo.com to request it.
Q.What immunogen was used to raise this antibody?
A.Recombinant mouse AGRP fragment (Ser82–Thr131), expressed in E. coli.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Zhou Xihong et al. (2018) Long-Term l-Serine Administration Reduces Food Intake and Improves Oxidative Stress and Sirt1/NFκB Signaling in the Hypothalamus of Aging Mice. Frontiers in Endocrinology. 10.3389/fendo.2018.00476

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