| Field | Specification |
|---|---|
| Target | |
| Alternative names | Type-2 angiotensin II receptor;Angiotensin II type-2 receptor;AT2;AGTR2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AGTR2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FIO-1 is a rabbit monoclonal antibody that recognizes type-2 angiotensin II receptor (gene AGTR2) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 41.2 kDa, and the supplier reports an observed band at about 45 kDa.
The angiotensin II type 2 receptor (AT2R, AGTR2) is a G protein-coupled receptor that signals mainly through non-canonical pathways. It often counterbalances AT1R, for example by limiting ERK activation and cell proliferation, and it is studied in cardiovascular, renal and developmental biology.
| Target | Type-2 angiotensin II receptor (gene AGTR2; UniProt P50052, human) |
|---|---|
| Clone | FIO-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.2 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human HepG2 cells, human SH-SY5Y cells, human Caco-2 cells, rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human AGTR2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places type-2 angiotensin II receptor in the cell membrane. Tissue expression noted by UniProt (human): In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ying-Shuang Chang et al. (2022) Exercise Normalized the Hippocampal Renin-Angiotensin System and Restored Spatial Memory Function, Neurogenesis, and Blood-Brain Barrier Permeability in the 2K1C-Hypertensive Mouse. International Journal of Molecular Sciences. 10.3390/ijms23105531
Larissa M. Macedo et al. (2020) Effect of angiotensin II and angiotensin-(1–7) on proliferation of stem cells from human dental apical papilla. Journal of Cellular Physiology. 10.1002/jcp.29862
Gomes Karina Pereira et al. (2020) Antiepileptic effects of long-term intracerebroventricular infusion of angiotensin-(1-7) in an animal model of temporal lobe epilepsy. Clinical Science. 10.1042/CS20200514
Fei Qian et al. (2025) Chlorogenic Acid Ameliorates Chronic Unpredictable Stress-Induced Diminished Ovarian Reserve Through Ovarian Renin-Angiotensin System. Molecular Nutrition & Food Research. 10.1002/mnfr.202400814
